ISO INTERNATIONAL STANDARD 6225-1 Second edition 2006-10-01 Rubber, raw, natural Determination of castor oil content - Part 1: Determination of castor oil glyceride content by thin-layer chromatography CaoutchoucnaturelbrutDeterminationde lateneurenhuilede ricin- Partie 1:Determination de la teneur en glycerides d'huile de ricin par chromatographieencouchemince Reference number ISO 6225-1:2006(E) @ISO2006 ISO6225-1:2006(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. @ISO2006 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, Iso's memberbody in the country of the requester. ISO copyright office Case postale 56 : CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland ISO 6225-1:2006(E) Foreword IsO (the International Organization for Standardization)is a worldwide federation of national standards bodies non-governmental, in liaison with isO, also take part in the work. iso collaborates closely with the International Electrotechnical Commission (IEC)on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO6225-1wasprepared byTechnical CommitteeISO/TC 45,Rubberand rubberproducts,Subcommittee Sc 2,Testing and analysis. update the normative references. castoroilcontent: Part1:Determinationofcastoroilglyceridecontentbythin-layerchromatography Part 2:Determination of total ricinoleic acid contentby gas chromatography ISO6225-1:2006(E) Introduction Certain grades of natural rubber are treated with castor oil to facilitate crumbing of the rubber during the rubber. The principal constituent of castor oil, making up about 80 % to 85 % (by mass), is the triglyceride of ricinoleic acid. This glyceride may partly hydrolyse to ricinoleic acid and glycerol. ISO2006-Allrightsreserved ISO 6225-1:2006(E) INTERNATIONALSTANDARD Rubber, raw, natural Determination of castor oil content - Part 1: Determination of castor oil glyceride content by thin-layer chromatography WARNING-Personsusing this part of ISo6225 should befamiliarwithnormal laboratorypractice. use. It is the responsibility of the user to establish appropriate safety and health practices and to ensurecompliancewithany nationalregulatoryconditions. 1 Scope This part of Iso 6225 specifies a thin-layer chromatographic method for the determination of the castor oil and castor oil glyceride content of raw rubber. It is applicable to all grades of natural rubber. The lower limit of detection is approximately 0,05 % of castor oil glycerides. 2Normativereferences The following referenced documents are indispensable for the application of